H00003911-D01P

antibody from Abnova Corporation

Targeting: LAMA5

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003911-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003911-D01P, RRID:AB_1576748

Product name

LAMA5 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human LAMA5 protein.

Antigen sequence

MGVSLRDKKVHWVYQLGEAGPAVLSIDEDIGEQFA
AVSLDRTLQFGHMSVTVERQMIQETKGDTVAPGAE
GLLNLRPDDFVFYVGGYPSTFTPPPLLRFPGYRGC
IEMDTLNEEVVSLYNFERTFQLDTAVDRPCARSKS
TGDPWLTDGSYLDGTGFARISFDSQISTTKRFEQE
LRLVSYSGVLFFLKQQIQVFLLGGSRKRVLVRVER
ATVYSVEQDNDLELADAYYLGGVPPDQLPPSLRRL
FPTGGSVRGCVKGIKALGKYVDLKRLNTTGVSAGC
TADLLVGRAMTFHGHGFLRLALSNVAPLTGNVYSG
FGFHSAQDSALLYYRASPDGLCQVSLQQGRVSLQL
LRTEVKTQAGFADGAPHYVAFYSNATGVWLYVDDQ
LQQMKPHRGPPPELQPQPEGPPRLLLGGLPESGTI
YNFSGCISNVFVQRLLGPQRVFDLQQNLGSVNVST
GCAPALQAQTPGLGPRGLQATARKASRRSRQPARH
PACMLPPHLRTTRDSYQFGGSLSSHLEFVGILARH
RNWPSLSMHVLPRSSRGLLLFTARLRPGSPSLALF
LSNGHFVAQMEGLGTRLRAQSRQRSRPGRWHKVSV
RWEKNRILLVTDGARAWSQEGPHRQHQGAEHPQPH
TLFVGGLPASSHSSKLPTSQELHCLMWAWNWRCGP
WQSPD


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of LAMA5 expression in transfected 293T cell line (H00003911-T01) by LAMA5 MaxPab polyclonal antibody.Lane 1: LAMA5 transfected lysate(74.00 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between LAMA5 and LAMC1. HeLa cells were stained with anti-LAMA5 rabbit purified polyclonal 1:1200 and anti-LAMC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)