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Western blotAntibody data
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- Antigen structure
- References [1]
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- Western blot [3]
- Immunohistochemistry [5]
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- Product number
- MA5-24649 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Laminin alpha-5 Monoclonal Antibody (CL3118)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LNLSSIILDV NQDRLTQRAI EASNAYSRIL QAVQAAEDAA GQALQQADHT WATVVRQGLV DRAQQLLANS TALEEAMLQE QQRLGLVWAA L
- Antibody clone number
- CL3118
- Concentration
- 1 mg/mL
Submitted references The microvascular extracellular matrix in brains with Alzheimer's disease neuropathologic change (ADNC) and cerebral amyloid angiopathy (CAA).
Damodarasamy M, Vernon RB, Pathan JL, Keene CD, Day AJ, Banks WA, Reed MJ
Fluids and barriers of the CNS 2020 Sep 29;17(1):60
Fluids and barriers of the CNS 2020 Sep 29;17(1):60
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin alpha-5 in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Laminin alpha-5 Monoclonal Antibody (CL3118) (Product # MA5-24649). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin alpha-5 in human placenta tissue lysates using Laminin alpha-5 Monoclonal Antibody (CL3118) (Product # MA5-24649).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of purified human recombinant Laminin-511, Laminin-521, Laminin-121, Laminin-221, Laminin-332 and Laminin-411. Samples probed using a Laminin alpha-5 Monoclonal Antibody (CL3118) (Product # MA5-24649).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Laminin alpha-5 in human placenta and lymph node tissues using Laminin alpha-5 Monoclonal Antibody (CL3118) (Product # MA5-24649). Corresponding LAMA5 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Laminin alpha-5 in human kidney using Laminin alpha-5 Monoclonal Antibody (CL3118) (Product # MA5-24649) shows strong membranous positivity in glomeruli, as well as in basement membrane of renal tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Laminin alpha-5 in human placenta using Laminin alpha-5 Monoclonal Antibody (CL3118) (Product # MA5-24649) shows moderate positivity in basement membrane of trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Laminin alpha-5 in human colon using Laminin alpha-5 Monoclonal Antibody (CL3118) (Product # MA5-24649) shows moderate positivity in basement membrane of glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Laminin alpha-5 in human lymph node using Laminin alpha-5 Monoclonal Antibody (CL3118) (Product # MA5-24649) shows no positivity in lymphoid cells as expected.
