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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00003911-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003911-M01, RRID:AB_540114
- Product name
- Mouse Monoclonal Laminin alpha 5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. LAMA5 - laminin, alpha 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Integrin-Mediated Macrophage Adhesion Promotes Lymphovascular Dissemination in Breast Cancer.
Two-Color Lateral Flow Assay for Multiplex Detection of Causative Agents Behind Acute Febrile Illnesses.
Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion.
Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors.
Proteomics analysis of nasopharyngeal carcinoma cell secretome using a hollow fiber culture system and mass spectrometry.
Proteomic analysis of platelet alpha-granules using mass spectrometry.
Evans R, Flores-Borja F, Nassiri S, Miranda E, Lawler K, Grigoriadis A, Monypenny J, Gillet C, Owen J, Gordon P, Male V, Cheung A, Noor F, Barber P, Marlow R, Francesch-Domenech E, Fruhwirth G, Squadrito M, Vojnovic B, Tutt A, Festy F, De Palma M, Ng T
Cell reports 2019 May 14;27(7):1967-1978.e4
Cell reports 2019 May 14;27(7):1967-1978.e4
Two-Color Lateral Flow Assay for Multiplex Detection of Causative Agents Behind Acute Febrile Illnesses.
Lee S, Mehta S, Erickson D
Analytical chemistry 2016 Sep 6;88(17):8359-63
Analytical chemistry 2016 Sep 6;88(17):8359-63
Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion.
Little GH, Noushmehr H, Baniwal SK, Berman BP, Coetzee GA, Frenkel B
Nucleic acids research 2012 Apr;40(8):3538-47
Nucleic acids research 2012 Apr;40(8):3538-47
Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors.
Oikawa Y, Hansson J, Sasaki T, Rousselle P, Domogatskaya A, Rodin S, Tryggvason K, Patarroyo M
Experimental cell research 2011 May 1;317(8):1119-33
Experimental cell research 2011 May 1;317(8):1119-33
Proteomics analysis of nasopharyngeal carcinoma cell secretome using a hollow fiber culture system and mass spectrometry.
Wu HY, Chang YH, Chang YC, Liao PC
Journal of proteome research 2009 Jan;8(1):380-9
Journal of proteome research 2009 Jan;8(1):380-9
Proteomic analysis of platelet alpha-granules using mass spectrometry.
Maynard DM, Heijnen HF, Horne MK, White JG, Gahl WA
Journal of thrombosis and haemostasis : JTH 2007 Sep;5(9):1945-55
Journal of thrombosis and haemostasis : JTH 2007 Sep;5(9):1945-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Laminin alpha 5 Antibody (2F7) [H00003911-M01] - Analysis of LAMA5 expression in transfected 293T cell line by LAMA5 monoclonal antibody (M01), clone 2F7.Lane 1: LAMA5 transfected lysate(74 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Laminin alpha 5 Antibody (2F7) [H00003911-M01] - Detection limit for recombinant GST tagged LAMA5 is approximately 0.03ng/ml as a capture antibody.
