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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-32183 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAAH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: MCF-7. Predicted reactivity: Mouse (84%), Rat (82%), Pig (85%), Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FAAH Polyclonal Antibody (Product # PA5-32183). Sample (30 µg of whole cell lysate). Lane A: MCF-7 . 7.5% SDS PAGE. FAAH Polyclonal Antibody (Product # PA5-32183) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of LNCaP (Lane 1), PC-3 (Lane 2), DU 145 (Lane 3), MCF7 (Lane 4), A-431 (Lane 5), HEK 293 (Lane 6), tissue extracts of Mouse Liver (Lane 7) and Rat Liver (Lane 8). The blot was probed with Anti- FAAH Polyclonal Antibody (Product # PA5-32183, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 60 kDa band corresponding to FAAH was detected across cell lines tested except PC-3 and DU 145 which are reported to be negative for FAAH expression in comparison to LNCaP cell line.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using FAAH (Product # PA5-32183) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
