10322-1-AP

antibody from Proteintech Group

Targeting: NUDT21 CFIM25, CPSF5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

10322-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10322-1-AP, RRID:AB_2251496

Product name

NUDT21 antibody

Antibody type

Polyclonal

Description

KD/KO validated NUDT21 antibody (Cat. #10322-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Transposable element-gene chimera cartography, origination and role in enhancing transcriptome plasticity.

Cheon Y, Alvstad EG, Torre D, Quach DT, Nguyen J, Hyun K, Zhou M, Yu T, Liu L, Yoon Y, Reese F, Faraone L, Li Y, Arnold FJ, Fstkchyan YS, Basu U, Kvon E, Valente EM, Ho JSY, Byun M, Guccione E, Shi Y, Weng Z, Seldin M, Marazzi I
Nature structural & molecular biology 2026 Mar;33(3):448-463

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3'UTR shortening alleviates miRNA repression of mRNAs critical for muscle stem cell differentiation.

Zhu Y, Wang J, Tong D, Jia P, Chen S, Li Y, Fu J, Li Q, Hu P, Zhou Y, Cheng H
The EMBO journal 2026 Feb;45(3):722-748

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NUDT21-mediated Alternative Polyadenylation of CDK19 Reprograms Cholesterol Biosynthesis to Drive Colorectal Cancer Progression.

Yu Y, Ma J, Zhou M, Gu X, Zhou Y, Li Z, Zhang T, Gong W, Huang C, Xiang J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Feb;13(7):e18346

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SNW1 promotes lymphatic metastasis in bladder cancer by modulating SRPK1 splicing.

Hu J, Zhu Y, Wang G, Dai H, Han H, Cao W, Ding M, Diao W, Lu Q, Zhang Q, Guo H, Chen W
iScience 2026 Feb 20;29(2):114811

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Alternative Polyadenylation Contributes to Fibroblast Senescence in Pulmonary Fibrosis.

Huang J, Gacha-Garay MJ, Wang Y, Collum SD, Girard RA, Puente H, Yoo SH, Hussain R, Patel J, Patel M, Wagner EJ, KrishnaYalamanchili H, Karmouty-Quintana H, Chen Z, Mills T
Aging cell 2025 Oct;24(10):e70179

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NUDT21 lactylation reprograms alternative polyadenylation to promote cuproptosis resistance.

Lin J, Yin Y, Cao J, Zhang Y, Chen J, Chen R, Zou B, Huang C, Lv Y, Xu S, Yang H, Lin P, Xie D
Cell discovery 2025 May 28;11(1):52

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RNA polymerase III transcription-associated polyadenylation promotes the accumulation of noncoding retrotransposons during infection.

Lari A, Shah SB, Mao X, Sanghrajka P, Karijolich J, Lareau LF, Glaunsinger BA
bioRxiv : the preprint server for biology 2025 May 15;

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Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors.

Lu C, Bai X, Zhang H, Zhang Y, Yang M, Zheng Y, Jin Z, Yang W, Guo G, Huang Q, Huang Y, Wu L, Fu XD, Zhang Z, Wang G
Molecular cell 2025 Jun 5;85(11):2147-2164.e10

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IGF2BP2 binding to CPSF6 facilitates m6A-mediated alternative polyadenylation of PUM2 and promotes malignant progression in ovarian cancer.

Luo X, Wei Q, Xie L, Chen N, Gu B, Xu J, Jiang X, Zuo X, Zhao H, Liu X, Yang Y, Liu T, Zhu Y, Yi P, Xu J
Clinical and translational medicine 2025 Jul;15(7):e70388

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Genetic Regulation of Alternative Polyadenylation Provides Novel Insights into Molecular Mechanisms Underlying Non-small Cell Lung Cancer.

Jin M, Huang Y, Li B, Wang Y, Li Y, Chen Z, Tang Z, Liu C, Zhang L, Yuan X, Tian J, Liu B
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jul;12(26):e2502008

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Characterization and molecular targeting of CFIm25 (NUDT21/CPSF5) mRNA using miRNAs.

Khan N, Gupta M, Masamha CP
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jan 31;39(2):e70324

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E3 ligase SYVN1-mediated polyubiquitination of CPSF6 promotes alternative polyadenylation and antivirus effects of macrophages.

Lu X, Liu C, Wu R, Hu Z, Liu S, Li X, Liu Y, Li M, Liang J, Huang Y, Han Y, Ou X, Deng K, Liang C, Chen S, Fu Y, Xu A
Cell reports 2025 Feb 25;44(2):115276

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CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.

Liang S, Zhu Z, Tang Y, Zhou S, Xiao M, Gong X, Ye K
International journal of molecular medicine 2025 Dec;56(6)

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Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells.

Cornish D, Jackson-Jones KA, Ling-Hu T, Simons LM, Cisneros WJ, Kuffour EO, Stegman N, Agnes F, Lee Y, Sharma VP, Bieniasz PD, Lorenzo-Redondo R, Hultquist JF
PLoS pathogens 2025 Dec;21(12):e1013745

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RNA polymerase III transcription-associated polyadenylation promotes the accumulation of noncoding retrotransposons during infection.

Lari A, Shah SB, Mao X, Sanghrajka P, Karijolich J, Lareau LF, Glaunsinger BA
Proceedings of the National Academy of Sciences of the United States of America 2025 Aug 12;122(32):e2507186122

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IGF2BP3 recruits NUDT21 to regulate SPTBN1 alternative polyadenylation and drive ovarian cancer progression.

Luo X, Xie L, Ma X, Chen N, Xu J, Jiang X, Liu X, Yang Y, Liu T, Yi P, Xu J
Communications biology 2025 Apr 29;8(1):680

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Unraveling the role of MiR-181 in skin fibrosis pathogenesis by targeting NUDT21.

Mills TW, Wu M, Alonso J, Puente H, Charles J, Chen Z, Yoo SH, Mayes MD, Assassi S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Sep;38(17):e70022

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TRIM65 deficiency alleviates renal fibrosis through NUDT21-mediated alternative polyadenylation.

Wei S, Huang X, Zhu Q, Chen T, Zhang Y, Tian J, Pan T, Zhang L, Xie T, Zhang Q, Kuang X, Lei E, Li Y
Cell death and differentiation 2024 Nov;31(11):1422-1438

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RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation.

Lin P, Chen W, Long Z, Yu J, Yang J, Xia Z, Wu Q, Min X, Tang J, Cui Y, Liu F, Wang C, Zheng J, Li W, Rich JN, Li L, Xie Q
Cell discovery 2024 Mar 19;10(1):32

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Downregulation of CPSF6 leads to global mRNA 3' UTR shortening and enhanced antiviral immune responses.

Ge Y, Huang J, Chen R, Fu Y, Ling T, Ou X, Rong X, Cheng Y, Lin Y, Zhou F, Lu C, Yuan S, Xu A
PLoS pathogens 2024 Feb;20(2):e1012061

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Nudt21-mediated alternative polyadenylation of MZT1 3'UTR contributes to pancreatic cancer progression.

Zhou Y, Yang J, Huang L, Liu C, Yu M, Chen R, Zhou Q
iScience 2024 Feb 16;27(2):108822

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Alternative mRNA polyadenylation regulates macrophage hyperactivation via the autophagy pathway.

Chen Y, Chen B, Li J, Li H, Wang G, Cai X, Zhang Q, Liu X, Kan C, Wang L, Wang Z, Li HB
Cellular & molecular immunology 2024 Dec;21(12):1522-1534

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Disruption of CPSF6 enhances cellular permissivity to HIV-1 infection through alternative polyadenylation.

Hultquist J, Cornish D, Jackson-Jones K, Ling-Hu T, Simons L, Cisneros W, Kuffour E, Agnes F, Lee Y, Bieniasz P, Lorenzo-Redondo R
Research square 2024 Dec 4;

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Dynamic regulation of alternative polyadenylation by PQBP1 during neurogenesis.

Liu X, Xie H, Liu W, Zuo J, Li S, Tian Y, Zhao J, Bai M, Li J, Bao L, Han J, Zhang ZC
Cell reports 2024 Aug 27;43(8):114525

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Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis.

Chen X, Shi W, Zhu L, Zhou X, Wang Y
Cellular signalling 2023 Sep;109:110752

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CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation.

Liu S, Wu R, Chen L, Deng K, Ou X, Lu X, Li M, Liu C, Chen S, Fu Y, Xu A
Cell reports 2023 Oct 31;42(10):113197

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Synergistic inhibition of NUDT21 by secretory S100A11 and exosomal miR-487a-5p promotes melanoma oligo- to poly-metastatic progression.

Zeng B, Chen Y, Chen H, Zhao Q, Sun Z, Liu D, Li X, Zhang Y, Wang J, Xing HR
Molecular oncology 2023 Dec;17(12):2743-2766

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CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation.

Ran Y, Huang S, Shi J, Feng Q, Deng Y, Xiang AP, Yao C
RNA biology 2022;19(1):686-702

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Distinct, opposing functions for CFIm59 and CFIm68 in mRNA alternative polyadenylation of Pten and in the PI3K/Akt signalling cascade.

Tseng HW, Mota-Sydor A, Leventis R, Jovanovic P, Topisirovic I, Duchaine TF
Nucleic acids research 2022 Sep 9;50(16):9397-9412

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NUDT21 Promotes Tumor Growth and Metastasis Through Modulating SGPP2 in Human Gastric Cancer.

Zhu Y, Zhang R, Zhang Y, Cheng X, Li L, Wu Z, Ding K
Frontiers in oncology 2021;11:670353

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Alternative polyadenylation is a determinant of oncogenic Ras function.

Subramanian A, Hall M, Hou H, Mufteev M, Yu B, Yuki KE, Nishimura H, Sathaseevan A, Lant B, Zhai B, Ellis J, Wilson MD, Daugaard M, Derry WB
Science advances 2021 Dec 17;7(51):eabh0562

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NUDT21 Suppresses Breast Cancer Tumorigenesis Through Regulating CPSF6 Expression.

Wang BJ, Liu DC, Guo QY, Han XW, Bi XM, Wang H, Wu ZS, Wu WY
Cancer management and research 2020;12:3069-3078

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ARS2 Regulates Nuclear Paraspeckle Formation through 3'-End Processing and Stability of NEAT1 Long Noncoding RNA.

Machitani M, Taniguchi I, Ohno M
Molecular and cellular biology 2020 Jan 30;40(4)

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Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation.

Weng T, Huang J, Wagner EJ, Ko J, Wu M, Wareing NE, Xiang Y, Chen NY, Ji P, Molina JG, Volcik KA, Han L, Mayes MD, Blackburn MR, Assassi S
The Journal of experimental medicine 2020 Feb 3;217(2)

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CFIm25-regulated lncRNA acv3UTR promotes gastric tumorigenesis via miR-590-5p/YAP1 axis.

Liu K, Wang BJ, Han W, Chi CH, Gu C, Wang Y, Fu X, Huang W, Liu Z, Song X
Oncogene 2020 Apr;39(15):3075-3088

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Partial loss of CFIm25 causes learning deficits and aberrant neuronal alternative polyadenylation.

Alcott CE, Yalamanchili HK, Ji P, van der Heijden ME, Saltzman A, Elrod N, Lin A, Leng M, Bhatt B, Hao S, Wang Q, Saliba A, Tang J, Malovannaya A, Wagner EJ, Liu Z, Zoghbi HY
eLife 2020 Apr 22;9

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Transforming growth factor β1 alters the 3'-UTR of mRNA to promote lung fibrosis.

Ko J, Mills T, Huang J, Chen NY, Mertens TCJ, Collum SD, Lee G, Xiang Y, Han L, Zhou Y, Lee CG, Elias JA, Jyothula SSK, Rajagopal K, Karmouty-Quintana H, Blackburn MR
The Journal of biological chemistry 2019 Oct 25;294(43):15781-15794

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A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.

So BR, Di C, Cai Z, Venters CC, Guo J, Oh JM, Arai C, Dreyfuss G
Molecular cell 2019 Nov 21;76(4):590-599.e4

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Cleavage factor 25 deregulation contributes to pulmonary fibrosis through alternative polyadenylation.

Weng T, Ko J, Masamha CP, Xia Z, Xiang Y, Chen NY, Molina JG, Collum S, Mertens TC, Luo F, Philip K, Davies J, Huang J, Wilson C, Thandavarayan RA, Bruckner BA, Jyothula SS, Volcik KA, Li L, Han L, Li W, Assassi S, Karmouty-Quintana H, Wagner EJ, Blackburn MR
The Journal of clinical investigation 2019 May 1;129(5):1984-1999

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THZ1 suppresses human non-small-cell lung cancer cells in vitro through interference with cancer metabolism.

Cheng ZJ, Miao DL, Su QY, Tang XL, Wang XL, Deng LB, Shi HD, Xin HB
Acta pharmacologica Sinica 2019 Jun;40(6):814-822

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The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.

Chen S, Wang R, Zheng D, Zhang H, Chang X, Wang K, Li W, Fan J, Tian B, Cheng H
Molecular cell 2019 Apr 4;74(1):118-131.e7

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Suppression of cleavage factor Im 25 promotes the proliferation of lung cancer cells through alternative polyadenylation.

Huang J, Weng T, Ko J, Chen NY, Xiang Y, Volcik K, Han L, Blackburn MR, Lu X
Biochemical and biophysical research communications 2018 Sep 5;503(2):856-862

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Effect of CFIm25 knockout on RNA polymerase II transcription.

Tellier M, Hardy JG, Norbury CJ, Murphy S
BMC research notes 2018 Dec 14;11(1):894

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CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.

Masamha CP, Xia Z, Peart N, Collum S, Li W, Wagner EJ, Shyu AB
RNA (New York, N.Y.) 2016 Jun;22(6):830-8

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Coupling between alternative polyadenylation and alternative splicing is limited to terminal introns.

Movassat M, Crabb TL, Busch A, Yao C, Reynolds DJ, Shi Y, Hertel KJ
RNA biology 2016 Jul 2;13(7):646-55

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Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors.

Misra A, Ou J, Zhu LJ, Green MR
Molecular cell 2015 Jun 4;58(5):819-31

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The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination.

Yao X, Tang Z, Fu X, Yin J, Liang Y, Li C, Li H, Tian Q, Roeder RG, Wang G
The EMBO journal 2015 Dec 2;34(23):2885-902

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CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Masamha CP, Xia Z, Yang J, Albrecht TR, Li M, Shyu AB, Li W, Wagner EJ
Nature 2014 Jun 19;510(7505):412-6

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Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation.

Kim S, Yamamoto J, Chen Y, Aida M, Wada T, Handa H, Yamaguchi Y
Genes to cells : devoted to molecular & cellular mechanisms 2010 Sep 1;15(9):1003-13

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Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis.

Sartini BL, Wang H, Wang W, Millette CF, Kilpatrick DL
Biology of reproduction 2008 Mar;78(3):472-82

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 10322-1-AP(NUDT21 antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using NUDT21 antibody 10322-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human placenta using 10322-1-AP(NUDT21 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human placenta using 10322-1-AP(NUDT21 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue