Antibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 10322-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10322-1-AP, RRID:AB_2251496
- Product name
- NUDT21 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated NUDT21 antibody (Cat. #10322-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation.
Downregulation of CPSF6 leads to global mRNA 3' UTR shortening and enhanced antiviral immune responses.
Nudt21-mediated alternative polyadenylation of MZT1 3'UTR contributes to pancreatic cancer progression.
Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis.
CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation.
Synergistic inhibition of NUDT21 by secretory S100A11 and exosomal miR-487a-5p promotes melanoma oligo- to poly-metastatic progression.
CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation.
Distinct, opposing functions for CFIm59 and CFIm68 in mRNA alternative polyadenylation of Pten and in the PI3K/Akt signalling cascade.
NUDT21 Promotes Tumor Growth and Metastasis Through Modulating SGPP2 in Human Gastric Cancer.
Alternative polyadenylation is a determinant of oncogenic Ras function.
NUDT21 Suppresses Breast Cancer Tumorigenesis Through Regulating CPSF6 Expression.
ARS2 Regulates Nuclear Paraspeckle Formation through 3'-End Processing and Stability of NEAT1 Long Noncoding RNA.
Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation.
CFIm25-regulated lncRNA acv3UTR promotes gastric tumorigenesis via miR-590-5p/YAP1 axis.
Partial loss of CFIm25 causes learning deficits and aberrant neuronal alternative polyadenylation.
Transforming growth factor β1 alters the 3'-UTR of mRNA to promote lung fibrosis.
A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.
Cleavage factor 25 deregulation contributes to pulmonary fibrosis through alternative polyadenylation.
THZ1 suppresses human non-small-cell lung cancer cells in vitro through interference with cancer metabolism.
The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.
Suppression of cleavage factor Im 25 promotes the proliferation of lung cancer cells through alternative polyadenylation.
Effect of CFIm25 knockout on RNA polymerase II transcription.
CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.
Coupling between alternative polyadenylation and alternative splicing is limited to terminal introns.
Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors.
The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination.
CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.
Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation.
Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis.
Lin P, Chen W, Long Z, Yu J, Yang J, Xia Z, Wu Q, Min X, Tang J, Cui Y, Liu F, Wang C, Zheng J, Li W, Rich JN, Li L, Xie Q
Cell discovery 2024 Mar 19;10(1):32
Cell discovery 2024 Mar 19;10(1):32
Downregulation of CPSF6 leads to global mRNA 3' UTR shortening and enhanced antiviral immune responses.
Ge Y, Huang J, Chen R, Fu Y, Ling T, Ou X, Rong X, Cheng Y, Lin Y, Zhou F, Lu C, Yuan S, Xu A
PLoS pathogens 2024 Feb;20(2):e1012061
PLoS pathogens 2024 Feb;20(2):e1012061
Nudt21-mediated alternative polyadenylation of MZT1 3'UTR contributes to pancreatic cancer progression.
Zhou Y, Yang J, Huang L, Liu C, Yu M, Chen R, Zhou Q
iScience 2024 Feb 16;27(2):108822
iScience 2024 Feb 16;27(2):108822
Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis.
Chen X, Shi W, Zhu L, Zhou X, Wang Y
Cellular signalling 2023 Sep;109:110752
Cellular signalling 2023 Sep;109:110752
CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation.
Liu S, Wu R, Chen L, Deng K, Ou X, Lu X, Li M, Liu C, Chen S, Fu Y, Xu A
Cell reports 2023 Oct 31;42(10):113197
Cell reports 2023 Oct 31;42(10):113197
Synergistic inhibition of NUDT21 by secretory S100A11 and exosomal miR-487a-5p promotes melanoma oligo- to poly-metastatic progression.
Zeng B, Chen Y, Chen H, Zhao Q, Sun Z, Liu D, Li X, Zhang Y, Wang J, Xing HR
Molecular oncology 2023 Dec;17(12):2743-2766
Molecular oncology 2023 Dec;17(12):2743-2766
CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation.
Ran Y, Huang S, Shi J, Feng Q, Deng Y, Xiang AP, Yao C
RNA biology 2022;19(1):686-702
RNA biology 2022;19(1):686-702
Distinct, opposing functions for CFIm59 and CFIm68 in mRNA alternative polyadenylation of Pten and in the PI3K/Akt signalling cascade.
Tseng HW, Mota-Sydor A, Leventis R, Jovanovic P, Topisirovic I, Duchaine TF
Nucleic acids research 2022 Sep 9;50(16):9397-9412
Nucleic acids research 2022 Sep 9;50(16):9397-9412
NUDT21 Promotes Tumor Growth and Metastasis Through Modulating SGPP2 in Human Gastric Cancer.
Zhu Y, Zhang R, Zhang Y, Cheng X, Li L, Wu Z, Ding K
Frontiers in oncology 2021;11:670353
Frontiers in oncology 2021;11:670353
Alternative polyadenylation is a determinant of oncogenic Ras function.
Subramanian A, Hall M, Hou H, Mufteev M, Yu B, Yuki KE, Nishimura H, Sathaseevan A, Lant B, Zhai B, Ellis J, Wilson MD, Daugaard M, Derry WB
Science advances 2021 Dec 17;7(51):eabh0562
Science advances 2021 Dec 17;7(51):eabh0562
NUDT21 Suppresses Breast Cancer Tumorigenesis Through Regulating CPSF6 Expression.
Wang BJ, Liu DC, Guo QY, Han XW, Bi XM, Wang H, Wu ZS, Wu WY
Cancer management and research 2020;12:3069-3078
Cancer management and research 2020;12:3069-3078
ARS2 Regulates Nuclear Paraspeckle Formation through 3'-End Processing and Stability of NEAT1 Long Noncoding RNA.
Machitani M, Taniguchi I, Ohno M
Molecular and cellular biology 2020 Jan 30;40(4)
Molecular and cellular biology 2020 Jan 30;40(4)
Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation.
Weng T, Huang J, Wagner EJ, Ko J, Wu M, Wareing NE, Xiang Y, Chen NY, Ji P, Molina JG, Volcik KA, Han L, Mayes MD, Blackburn MR, Assassi S
The Journal of experimental medicine 2020 Feb 3;217(2)
The Journal of experimental medicine 2020 Feb 3;217(2)
CFIm25-regulated lncRNA acv3UTR promotes gastric tumorigenesis via miR-590-5p/YAP1 axis.
Liu K, Wang BJ, Han W, Chi CH, Gu C, Wang Y, Fu X, Huang W, Liu Z, Song X
Oncogene 2020 Apr;39(15):3075-3088
Oncogene 2020 Apr;39(15):3075-3088
Partial loss of CFIm25 causes learning deficits and aberrant neuronal alternative polyadenylation.
Alcott CE, Yalamanchili HK, Ji P, van der Heijden ME, Saltzman A, Elrod N, Lin A, Leng M, Bhatt B, Hao S, Wang Q, Saliba A, Tang J, Malovannaya A, Wagner EJ, Liu Z, Zoghbi HY
eLife 2020 Apr 22;9
eLife 2020 Apr 22;9
Transforming growth factor β1 alters the 3'-UTR of mRNA to promote lung fibrosis.
Ko J, Mills T, Huang J, Chen NY, Mertens TCJ, Collum SD, Lee G, Xiang Y, Han L, Zhou Y, Lee CG, Elias JA, Jyothula SSK, Rajagopal K, Karmouty-Quintana H, Blackburn MR
The Journal of biological chemistry 2019 Oct 25;294(43):15781-15794
The Journal of biological chemistry 2019 Oct 25;294(43):15781-15794
A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.
So BR, Di C, Cai Z, Venters CC, Guo J, Oh JM, Arai C, Dreyfuss G
Molecular cell 2019 Nov 21;76(4):590-599.e4
Molecular cell 2019 Nov 21;76(4):590-599.e4
Cleavage factor 25 deregulation contributes to pulmonary fibrosis through alternative polyadenylation.
Weng T, Ko J, Masamha CP, Xia Z, Xiang Y, Chen NY, Molina JG, Collum S, Mertens TC, Luo F, Philip K, Davies J, Huang J, Wilson C, Thandavarayan RA, Bruckner BA, Jyothula SS, Volcik KA, Li L, Han L, Li W, Assassi S, Karmouty-Quintana H, Wagner EJ, Blackburn MR
The Journal of clinical investigation 2019 May 1;129(5):1984-1999
The Journal of clinical investigation 2019 May 1;129(5):1984-1999
THZ1 suppresses human non-small-cell lung cancer cells in vitro through interference with cancer metabolism.
Cheng ZJ, Miao DL, Su QY, Tang XL, Wang XL, Deng LB, Shi HD, Xin HB
Acta pharmacologica Sinica 2019 Jun;40(6):814-822
Acta pharmacologica Sinica 2019 Jun;40(6):814-822
The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.
Chen S, Wang R, Zheng D, Zhang H, Chang X, Wang K, Li W, Fan J, Tian B, Cheng H
Molecular cell 2019 Apr 4;74(1):118-131.e7
Molecular cell 2019 Apr 4;74(1):118-131.e7
Suppression of cleavage factor Im 25 promotes the proliferation of lung cancer cells through alternative polyadenylation.
Huang J, Weng T, Ko J, Chen NY, Xiang Y, Volcik K, Han L, Blackburn MR, Lu X
Biochemical and biophysical research communications 2018 Sep 5;503(2):856-862
Biochemical and biophysical research communications 2018 Sep 5;503(2):856-862
Effect of CFIm25 knockout on RNA polymerase II transcription.
Tellier M, Hardy JG, Norbury CJ, Murphy S
BMC research notes 2018 Dec 14;11(1):894
BMC research notes 2018 Dec 14;11(1):894
CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.
Masamha CP, Xia Z, Peart N, Collum S, Li W, Wagner EJ, Shyu AB
RNA (New York, N.Y.) 2016 Jun;22(6):830-8
RNA (New York, N.Y.) 2016 Jun;22(6):830-8
Coupling between alternative polyadenylation and alternative splicing is limited to terminal introns.
Movassat M, Crabb TL, Busch A, Yao C, Reynolds DJ, Shi Y, Hertel KJ
RNA biology 2016 Jul 2;13(7):646-55
RNA biology 2016 Jul 2;13(7):646-55
Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors.
Misra A, Ou J, Zhu LJ, Green MR
Molecular cell 2015 Jun 4;58(5):819-31
Molecular cell 2015 Jun 4;58(5):819-31
The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination.
Yao X, Tang Z, Fu X, Yin J, Liang Y, Li C, Li H, Tian Q, Roeder RG, Wang G
The EMBO journal 2015 Dec 2;34(23):2885-902
The EMBO journal 2015 Dec 2;34(23):2885-902
CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.
Masamha CP, Xia Z, Yang J, Albrecht TR, Li M, Shyu AB, Li W, Wagner EJ
Nature 2014 Jun 19;510(7505):412-6
Nature 2014 Jun 19;510(7505):412-6
Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation.
Kim S, Yamamoto J, Chen Y, Aida M, Wada T, Handa H, Yamaguchi Y
Genes to cells : devoted to molecular & cellular mechanisms 2010 Sep 1;15(9):1003-13
Genes to cells : devoted to molecular & cellular mechanisms 2010 Sep 1;15(9):1003-13
Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis.
Sartini BL, Wang H, Wang W, Millette CF, Kilpatrick DL
Biology of reproduction 2008 Mar;78(3):472-82
Biology of reproduction 2008 Mar;78(3):472-82
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 10322-1-AP(NUDT21 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using NUDT21 antibody 10322-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human placenta using 10322-1-AP(NUDT21 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human placenta using 10322-1-AP(NUDT21 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue