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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-78352 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUDT21 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SK-N-SH, IMR32, SK-N-AS Predicted Reactivity: Zebrafish (98%), Xenopus laevis (98%), Pig (100%), Chicken (99%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.99 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NUDT21 in A) SK-N-SH whole cell lysate, B) IMR32 whole cell lysate, C) SK-N-AS whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with NUDT21 polyclonal antibody (Product # PA5-78352) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NUDT21 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NUDT21 Polyclonal Antibody (Product # PA5-78352) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NUDT21 Polyclonal Antibody detects NUDT21 protein at nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. NUDT21 Polyclonal Antibody (Product # PA5-78352) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NUDT21 Polyclonal Antibody detects NUDT21 protein at nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. NUDT21 Polyclonal Antibody (Product # PA5-78352) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NUDT21 Polyclonal Antibody detects NUDT21 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. NUDT21 Polyclonal Antibody (Product # PA5-78352) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
