Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-56239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MDGKDLSAGA VSAVQCIANP IKLARLVMEK TPHCFLTDQG AAQFAAAMGV PEIPGEKLVT ERNKKRLEKE KHEKGAQKTD CQ Highest antigen sequence identity to the following orthologs: Mouse - 77%, Rat - 79%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALP in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ALP Polyclonal Antibody (Product # PA5-56239).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ALP in human cell line U-2 OS shows positivity in microtubules & nucleus but excluded from the nucleoli. Samples were probed using an ALP Polyclonal Antibody (Product # PA5-56239).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human fallopian tube and skeletal muscle tissues using ALP Polyclonal Antibody (Product # PA5-56239). Corresponding ASRGL1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human cervix, uterine, endometrium, fallopian tube and skeletal muscle using ALP Polyclonal Antibody (Product # PA5-56239) (A) shows similar protein distribution across tissues to an independent ALP Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human testis using ALP Polyclonal Antibody (Product # PA5-56239) shows moderate positivity in a subset cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human skeletal muscle using ALP Polyclonal Antibody (Product # PA5-56239) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human fallopian tube using ALP Polyclonal Antibody (Product # PA5-56239) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human endometrium using ALP Polyclonal Antibody (Product # PA5-56239) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human cervix, uterine using ALP Polyclonal Antibody (Product # PA5-56239) shows moderate to strong cytoplasmic positivity in glandular cells.
