Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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- Product number
- PA5-63148 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ARLTLFHIEQ GKTVEEAADL SLGYMKSRVK GLGGLIVVSK TGDWVAKWTS TSMPWAAAKD GKLHFGIDPD DT Highest antigen sequence identity to the following orthologs: Mouse - 76%, Rat - 74%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALP in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a ALP Polyclonal Antibody (Product # PA5-63148). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALP in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ALP Polyclonal Antibody (Product # PA5-63148).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human skeletal muscle using ALP Polyclonal Antibody (Product # PA5-63148) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human fallopian tube and skeletal muscle tissues using ALP Polyclonal Antibody (Product # PA5-63148). Corresponding ASRGL1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human cervix, uterine, endometrium, fallopian tube and skeletal muscle using ALP Polyclonal Antibody (Product # PA5-63148) (A) shows similar protein distribution across tissues to an independent ALP Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human fallopian tube using ALP Polyclonal Antibody (Product # PA5-63148) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human skeletal muscle using ALP Polyclonal Antibody (Product # PA5-63148) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human endometrium using ALP Polyclonal Antibody (Product # PA5-63148) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALP in human cervix, uterine using ALP Polyclonal Antibody (Product # PA5-63148) shows moderate to strong cytoplasmic positivity in glandular cells.