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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C375656 - Provider product page
- Provider
- LSBio
- Product name
- ENTPD5 / CD39L4 Antibody (aa149-428) LS-C375656
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot. All lanes: ENTPD5 antibody at 4 ug/ml. Lane 1: mouse liver tissue. Lane 2: mouse kidney tissue. Lane 3: mouse gonad tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All lanes: ENTPD5 antibody at 4µg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Lane 3: Mouse gonad tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating ENTPD5 in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of ENTPD5 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: ENTPD5 Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ENTPD5 in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of ENTPD5 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: ENTPD5 Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
