Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-29245 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ENTPD5 Recombinant Rabbit Monoclonal Antibody (064)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human ENTPD5.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 64
- Vial size
- 100 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENTPD5 in Lane A: HepG2 Whole Cell Lysate (30 µg). Samples were probed using a ENTPD5 Monoclonal Antibody (Product # MA5-29245) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:47 kDa. Observed band size:53 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 53 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human ENTPD5 in human liver with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human ENTPD5 in human prostate with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ENTPD5 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 0.5 µL with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245) and 15 µL of 50 % Protein G agarose. Primary antibody: ENTPD5 Recombinant Rabbit Monoclonal Antibody (64), at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa.
