Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- MA5-29245 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ENTPD5 Recombinant Rabbit Monoclonal Antibody (64)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 64
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENTPD5 in Lane A: HepG2 Whole Cell Lysate (30 µg). Samples were probed using a ENTPD5 Monoclonal Antibody (Product # MA5-29245) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:47 kDa. Observed band size:53 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 53 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human ENTPD5 in human liver with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human ENTPD5 in human prostate with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ENTPD5 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 0.5 µL with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245) and 15 µL of 50 % Protein G agarose. Primary antibody: ENTPD5 Recombinant Rabbit Monoclonal Antibody (64), at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa.