MA5-34625

antibody from Invitrogen Antibodies

Targeting: SCGB1A1 CC10, CC16, CCSP, UGB

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34625

Provider

Invitrogen Antibodies

Product name

Uteroglobin Recombinant Rabbit Monoclonal Antibody (JU34-03)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: A549, A431, HUVEC, mouse lung tissue lysate.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JU34-03

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SCGB1A1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SCGB1A1 monoclonal antibody (Product # MA5-34625), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SCGB1A1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SCGB1A1 monoclonal antibody (Product # MA5-34625), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SCGB1A1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SCGB1A1 monoclonal antibody (Product # MA5-34625), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SCGB1A1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SCGB1A1 monoclonal antibody (Product # MA5-34625), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SCGB1A1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SCGB1A1 monoclonal antibody (Product # MA5-34625), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Uteroglobin with paraffin-embedded human trachea. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal Uteroglobin (Product # MA5-34625) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Uteroglobin with paraffin-embedded mouse lung. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal Uteroglobin (Product # MA5-34625) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SCGB1A1 in A549 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with SCGB1A1 monoclonal antibody (Product # MA5-34625) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SCGB1A1 in A549 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with SCGB1A1 monoclonal antibody (Product # MA5-34625) at a dilution of 1:100.