MA1-10050

antibody from Invitrogen Antibodies

Targeting: AHNAK MGC5395

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

Submit

Validation data

Product number: MA1-10050 - Provider product page
Provider: Invitrogen Antibodies
Product name: AHNAK Monoclonal Antibody (EM-09)
Antibody type: Monoclonal
Antigen: Other
Description: This antibody reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells. Immunocytochemistry: permeabilization is required.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: EM-09
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4° C, do not freeze

AHNAK protein structure - MA1-10050 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red) Monoclonal antibody (Product # MA1-10050). Cell nuclei stained with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of AHNAK was performed using 70% confluent log phase HaCaT cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with AHNAK Monoclonal Antibody (EM-09) (Product # MA1-10050, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell membrane and cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry staining of AHNAK1 in primary human fibroblasts using anti-AHNAK1 (EM-09; green) Monoclonal antibody (Product # MA1-10050). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).