- PA5-78171 - Invitrogen Antibodies FSCN1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Fascin 1 in wild-type and Fascin 1 knockout HeLa cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with Fascin 1 polyclonal antibody (Product # PA5-78171) using a dilution of 1:2000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Fascin 1 in HeLa cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with Fascin 1 polyclonal antibody (Product # PA5-78171) using a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fascin Polyclonal Antibody detects Fascin 1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract.10 % SDS-PAGE. Fascin Polyclonal Antibody (Product # PA5-78171) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of Fascin was achieved by transfecting SH-SY5Y with Fascin specific siRNAs (Silencer® select Product # s13208, s13207). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Fascin knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Fascin Polyclonal Antibody (Product # PA5-78171, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to Fascin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-Fascin Polyclonal Antibody(Product # PA5-78171) and a 54kDa band corresponding to Fascin was observed across all the tested cell lines and tissues, except U-87 MG and A549. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), U-87 MG (Lane 3), A549 (Lane 4), Neuro-2a (Lane 5), NTERA-2 cl.D1 (Lane 6), IMR-32 (Lane 7), Mouse Brain (Lane 8), Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of Fascin 1 in HCT116 cells. Samples were treated with 100% MeOH for 5 min and incubated with Fascin 1 polyclonal antibody (Product # PA5-78171) using a dilution of 1:500, followed by Hoechst.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FSCN1 antibody detects FSCN1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 100% MeOH for 5 min. Green: FSCN1 protein stained by FSCN1 antibody (Product # PA5-78171) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FSCN1 antibody detects FSCN1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 100% MeOH for 5 min. Green: FSCN1 protein stained by FSCN1 antibody (Product # PA5-78171) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fascin Polyclonal Antibody detects Fascin 1 protein at cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. Fascin Polyclonal Antibody (Product # PA5-78171) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-78171