NB100-79822

CPSF1 antibody from Novus Biologicals

CPSF160

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Data presented on provider website

Immunoprecipitation

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for NB100-79822

Antibody Data

Product number

NB100-79822

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-79822, RRID:AB_1107987

Product name

Rabbit Polyclonal CPSF1 Antibody

Provider product page

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CPSF1 Antibody [NB100-79822] - Detection of Human and Mouse CPSF160 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CPSF160 antibody NB100-79822 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CPSF160 was also immunoprecipitated by rabbit another anti-CPSF160 antibody, which recognizes an upstream epitope.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CPSF1 Antibody [NB100-79822] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CPSF160 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: CPSF1 Antibody [NB100-79822] - Detection of human CPSF160 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CPSF160 antibody NB100-79822 (lot NB100-79822-2) used for IP at 6 ug per reaction. CPSF160 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79822-1). For blotting immunoprecipitated CPSF160, NB100-79822 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:200 (1ug/ml).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB