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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- M01693 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Integrin alpha 6 ITGA6 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for CD49f/ITGA6 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human, Mouse, Rat CD49f/ITGA6 information: Molecular Weight: 126606 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Cell membrane ; Lipid-anchor ; Tissue Specificity: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- DHG-9
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice.
A three-dimensional biomimetic peripheral nerve model for drug testing and disease modelling.
Xu Z, Gong B, Li Z, Wang Y, Zhao Z, Xie L, Peng Y, Zhao S, Zhou H, Bian Y
Journal of cellular and molecular medicine 2023 Sep;27(18):2651-2660
Journal of cellular and molecular medicine 2023 Sep;27(18):2651-2660
A three-dimensional biomimetic peripheral nerve model for drug testing and disease modelling.
Malheiro A, Morgan F, Baker M, Moroni L, Wieringa P
Biomaterials 2020 Oct;257:120230
Biomaterials 2020 Oct;257:120230
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human uterus, using Integrin alpha 6 Antibody(M01693)ITGA6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGA6 Antibody (M01693)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
