PA5-47486
antibody from Invitrogen Antibodies
Targeting: CCL24
Ckb-6, eotaxin-2, MPIF-2, MPIF2, SCYA24
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-47486 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Eotaxin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse CCL24/Eotaxin-2/MPIF-2 is observed and less than 5% cross-reactivity with recombinant human (rh) MPIF-1 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Eotaxin 2 in immersion fixed paraffin-embedded sections of human skin. Samples were incubated in Eotaxin 2 polyclonal antibody (Product # PA5-47486) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of Eotaxin 2 in BaF3 mouse pro‚B cell line transfected with mouse CCR3. Samples were incubated in Eotaxin 2 polyclonal antibody (Product # PA5-47486). Recombinant Human CCL24/Eotaxin‚2 chemoattracts the BaF3 mouse pro‚B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL24/Eotaxin‚2 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL24/Eotaxin‚2 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.5-2.5 µg/mL.