500-P103G-100UG
antibody from Invitrogen Antibodies
Targeting: CCL24
Ckb-6, eotaxin-2, MPIF-2, MPIF2, SCYA24
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
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- Product number
- 500-P103G-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Eotaxin 2 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKGGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin-2 (CCL24). Anti-Human Eotaxin-2 (CCL24)-specific antibody was purified by affinity chromatography employing an immobilized Human Eotaxin-2 (CCL24) matrix. Sandwich ELISA: To detect Human Eotaxin-2 (CCL24) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Eotaxin-2 (CCL24) (500-P103GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin-2 (CCL24). Western Blot: To detect Human Eotaxin-2 (CCL24) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Eotaxin-2 (CCL24) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Goat
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.
Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5760-71
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5760-71
Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.
Mateo T, Abu Nabah YN, Abu Taha M, Mata M, Cerdá-Nicolás M, Proudfoot AE, Stahl RA, Issekutz AC, Cortijo J, Morcillo EJ, Jose PJ, Sanz MJ
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5577-86
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5577-86
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human Eotaxin-2 (CCL24) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of Eotaxin 2 Polyclonal Antibody (Product # 500-P103G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech Eotaxin 2 Polyclonal Antibody, Biotin (Product # 500-P103GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin-2 (CCL24).