Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-78192 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78192, RRID:AB_11019709
- Product name
- Rabbit Polyclonal ZNF462 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF462 Antibody [NBP1-78192] - Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-ZNF462 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ZNF462 was also immunoprecipitated by another rabbit anti-ZNF462 antibody which recognizes an upstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF462 Antibody [NBP1-78192] - Detection of Human ZNF462 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody NBP1-78192 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF462 Antibody [NBP1-78192] - NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZNF462 Antibody [NBP1-78192] - Detection of human ZNF462 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody NBP1-78192 (lot NBP1-78192-2) used for IP at 6 ug per reaction. ZNF462 was also immunoprecipitated by a previous lot of this antibody NBP1-78192-1. For blotting immunoprecipitated ZNF462, NBP1-78192 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZNF462 Antibody [NBP1-78192] - Section of human lung cancer. Antibody: Affinity purified rabbit anti- ZNF462 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.