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antibody from Invitrogen Antibodies
Targeting: ARHGEF7
BETA-PIX, COOL1, DKFZp686C12170, DKFZp761K1021, KIAA0142, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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- Product number
- PA5-52097 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARHGEF7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RMSGFIYQGK LPTTGMTITK LEDSENHRNA FEISGSMIER ILVSCNNQQD LQEWVEHLQK QTKVTSVGNP TIKPHSVPSH TLPSHPVTPS SKHADSKPAP LTPAYHTLPH PSHHGTPHTT INWGPLEPPK TPKPW
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARHGEF7 in human cell line SH-SY5Y using a ARHGEF7 Polyclonal Antibody (Product # PA5-52097).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KD of ARHGEF7 was achieved by transfecting A-431 with ARHGEF7 specific siRNAs (Silencer® select Product # S16948, S16949). Western blot analysis (Fig. a) was performed using whole cell extracts from the ARHGEF7 KD cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with ARHGEF7 Polyclonal Antibody (Product # PA5-52097, 0.4 µg/mL) and Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to ARHGEF7. An uncharacterized band was observed at ~32 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ARHGEF7 Polyclonal Antibody (Product # PA5-52097) and a 76 kDa band corresponding to ARHGEF7 was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Caco-2 (Lane 2), Daudi (Lane 3), A549 (Lane 4), HEK-293 (Lane 5), Hep G2 (Lane 6), SH-SY5Y (Lane 7) and tissue extract (30 µg lysate) of Mouse Lung (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band at ~32 kDa was also observed in certain samples tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARHGEF7 in human liver using ARHGEF7 Polyclonal Antibody (Product # PA5-52097) shows weak cytoplasmic positivity in hepatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARHGEF7 in human duodenum using ARHGEF7 Polyclonal Antibody (Product # PA5-52097) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARHGEF7 in human cerebellum using ARHGEF7 Polyclonal Antibody (Product # PA5-52097) shows moderate cytoplasmic positivity in Purkinje cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARHGEF7 in human cerebral cortex using ARHGEF7 Polyclonal Antibody (Product # PA5-52097) shows weak to moderate cytoplasmic positivity in neurons.
