14092-1-AP
antibody from Invitrogen Antibodies
Targeting: ARHGEF7
BETA-PIX, COOL1, DKFZp686C12170, DKFZp761K1021, KIAA0142, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 14092-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARHGEF7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: KELELQILT EAIRNWEGDD IKTLGNVTYM SQVLIQCAGS EEKNERYLLL FPNVLLMLSA SPRMSGFIYQ GKLPTTGMTI TKLEDSENHR NAFEISGSMI ERILVSCNNQ QDLQEWVEHL QKQTKVTSVG NPTIKPHSVP SHTLPSHPVT PSSKHADSKP APLTPAYHTL PHPSHHGTPH TTINWGPLEP PKTPKPWSLS CLRPAPPLRP SAALCYKEDL SKSPKTMKKL LPKRKPERKP SDEEFASRKS TAALEEDAQI LKVIEAYCTS AKTRQTLNST WQGTDLMHNH VLADDDQPSL DSLGRRSSLS RLEPSDLSED SDYDSIWTAH SYRMGSTSRK SCCSYISHQN (455-803 aa encoded by BC060776)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negatove control and ARHGEF7 overexpressed Transfected HEK-293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.