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LearnPA5-117482
antibody from Invitrogen Antibodies
Targeting: ARHGEF7
BETA-PIX, COOL1, DKFZp686C12170, DKFZp761K1021, KIAA0142, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-117482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARHGEF7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3.75 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of ARHGEF7 in HeLa cells using ARHGEF7 Polyclonal Antibody (Product # PA5-117482) diluted at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating ARHGEF7 in Hela whole cell lysate. Lane 1: Rabbit control IgG (1 µg) Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: ARHGEF7 Polyclonal Antibody (Product # PA5-117482) (6 µg) + Hela whole cell lysate (500 µg). Lane 3: Hela whole cell lysate (10 µg).
