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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- OAAB02948 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- ADH1C Antibody (Center) Affinity Purified Rabbit Polyclonal Antibody (0.1 mg)
- Antibody type
- Polyclonal
- Reactivity
- Human
- Vial size
- 0.1 mg
- Storage
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- ADH1C Antibody (Center) western blot analysis in T47D cell lysates (35ug/lane).This demonstrates the ADH1C antibody detected the ADH1C protein (arrow).
- Sample type
- T47D cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of ADH1C (arrow) using rabbit polyclonal ADH1C Antibody (Center) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the ADH1C gene.
- Sample type
- lysatestransiently transfected with the ADH1C gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- ADH1C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH1C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human hepatocarcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- ADH1C Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- CEM cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
