PA5-54105

antibody from Invitrogen Antibodies

Targeting: PPA1 IOPPP, PP, PP1, Ppase, SID6-8061

Antibody data

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Validation data

Product number: PA5-54105 - Provider product page
Provider: Invitrogen Antibodies
Product name: PPA1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: GFSTEERAAP FSLEYRVFLK NEKGQYISPF HDIPIYADKD VFHMVVEVPR WSNAK Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 91%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PPA1 protein structure - PA5-54105 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PPA1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a PPA1 Polyclonal Antibody (Product # PA5-54105).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PPA1 using PPA1 Polyclonal Antibody (Product # PA5-54105) (A) shows similar pattern to an independent PPA1 Polyclonal Antibody (B).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: KD of PPA1 was achieved by transfecting U-87 MG with PPA1 specific siRNAs (Silencer® select Product # s10878, s10877). Western blot analysis (Fig. a) was performed using whole cell extracts from the PPA1 KD cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PPA1 Polyclonal Antibody (Product # PA5-54105, 0.4µg/mL) and Goat Anti Rabbit (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PPA1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-PPA1 Rabbit Polyclonal Antibody (Product # PA5-54105) and 32 kDa band corresponding to PPA1 was observed across samples tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), U-87 MG (Lane 2), HT-29 (Lane 3), HCT 116 (Lane 4), SW-480 (Lane 5), MCF-7 (Lane 6), tissue extracts of Mouse Small Intestine (Lane 7) and Mouse Testis (Lane 8), were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4µg/mL) and detected by Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).