Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [9]
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Validation data
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- Product number
- LS-C782299 - Provider product page
- Provider
- LSBio
- Product name
- PPA1 Antibody LS-C782299
- Antibody type
- Polyclonal
- Description
- Antigen Affinity purification
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of human 1) PC-3, 2) Caco-2, 3) HEK293, 4) K562 and 5) U-2 OS lysate with PPA1 antibody at 0.5ug/ml. Predicted molecular weight: ~33 kDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of 1) rat thymus, 2) rat kidney, 3) rat testis, 4) mouse kidney and 5) mouse testis lysate with PPA1 antibody at 0.5ug/ml. Predicted molecular weight: ~33 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE human intestinal cancer with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE mouse brain with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human intestinal cancer with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain with PPA1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.