Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-48039 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- N-WASP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of DU145 human prostate carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human N-WASP Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48039) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for N-WASP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of N-WASP in DU145 human prostate carcinoma cell line, Neuro‚2A mouse neuroblastoma cell line, and PC‚12 rat adrenal pheochromocytoma cell line. Samples were incubated in N-WASP polyclonal antibody (Product # PA5-48039) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for N-WASP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of N-WASP in perfusion fixed frozen sections of rat brain (cortex) tissue. Samples were incubated in N-WASP polyclonal antibody (Product # PA5-48039) using a dilution of 15 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell cytoplasm.