NBP1-82512

antibody from Novus Biologicals

Targeting: WASL N-WASP, NWASP, WASPB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-82512

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-82512, RRID:AB_11045392

Product name

Rabbit Polyclonal N-WASP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human N-WASP antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Host-directed kinase inhibitors act as novel therapies against intracellular Staphylococcus aureus.

Bravo-Santano N, Stölting H, Cooper F, Bileckaja N, Majstorovic A, Ihle N, Mateos LM, Calle Y, Behrends V, Letek M
Scientific reports 2019 Mar 19;9(1):4876

---

Neural Wiskott-Aldrich syndrome protein (nWASP) is implicated in human lung cancer invasion.

Frugtniet BA, Martin TA, Zhang L, Jiang WG
BMC cancer 2017 Mar 28;17(1):224

---

N-wasp is required for stabilization of podocyte foot processes.

Schell C, Baumhakl L, Salou S, Conzelmann AC, Meyer C, Helmstädter M, Wrede C, Grahammer F, Eimer S, Kerjaschki D, Walz G, Snapper S, Huber TB
Journal of the American Society of Nephrology : JASN 2013 Apr;24(5):713-21

---

N-WASP coordinates the delivery and F-actin-mediated capture of MT1-MMP at invasive pseudopods.

Yu X, Zech T, McDonald L, Gonzalez EG, Li A, Macpherson I, Schwarz JP, Spence H, Futó K, Timpson P, Nixon C, Ma Y, Anton IM, Visegrády B, Insall RH, Oien K, Blyth K, Norman JC, Machesky LM
The Journal of cell biology 2012 Oct 29;199(3):527-44

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: N-WASP Antibody [NBP1-82512] - Simple Western lane view shows a specific band for N-WASP in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in human cell line RT-4.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human cerebral cortex shows moderate to strong positivity in neuronal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human kidney shows moderate to strong positivity in cells in tubules and glomeruli.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human skeletal muscle shows weak positivity in myocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human stomach shows moderate to strong positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding WASL RNA-seq data are presented for the same tissues.