Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- MA5-15011 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- N-WASP Monoclonal Antibody (C.427.3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- C.427.3
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of N-WASP in extracts from MCF-7 and Hela cells using N-WASP monoclonal antibody (Product # MA5-15011).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of WASL was achieved by transfecting MCF7 with WASL specific siRNAs (Silencer® select Product # s17133, s17132). Western blot analysis (Fig. a) was performed using whole cell extracts from the WASL knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with N-WASP Monoclonal Antibody (C.427.3) (Product # MA5-15011, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to WASL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using N-WASP Monoclonal Antibody (C.427.3) (Product # MA5-15011) and a~60 kDa band corresponding to WASL was observed in SkBr3, SHSY5Y, A431, MCF7 Mouse Lung and Mouse Cerebellum; and was absent in HEL 92.1.7, HL60 and Mouse skeletal Muscle. Whole cell extracts (30 µg lysate) of SkBr3 (Lane 1), SHSY5Y (Lane 2), A431 (Lane 3), MCF7 (Lane 4), HEL92.1.7 (Lane 5), HL60 (Lane 6), tissue extracts (30 µg lysate) of Mouse Lung (Lane 7), Mouse Cerebellum (Lane 8) and Mouse Skeletal Muscle (Lane 9) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).