HPA005750

antibody from Atlas Antibodies

Targeting: WASL N-WASP, NWASP, WASPB

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA005750

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005750, RRID:AB_1854729

Product name

Anti-WASL

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human WASL, Gene description: Wiskott-Aldrich syndrome-like, Alternative Gene Names: N-WASP, NWASP, Validated applications: ICC, WB, IHC, Uniprot ID: O00401, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin remodeling events in CD4 T cells

Sadhu L, Tsopoulidis N, Hasanuzzaman M, Laketa V, Way M, Fackler O
eLife 2023;12

---

Analysis of the distribution and expression of some tumor invasiveness markers in palate squamous cell carcinomas

Pătru A
Romanian Journal of Morphology and Embryology 2021;61(4):1259-1278

---

N-Wasp Regulates Oligodendrocyte Myelination

Katanov C, Novak N, Vainshtein A, Golani O, Dupree J, Peles E
The Journal of Neuroscience 2020;40(32):6103-6111

---

Microridges are apical epithelial projections formed of F-actin networks that organize the glycan layer

Pinto C, Khandekar A, Bhavna R, Kiesel P, Pigino G, Sonawane M
Scientific Reports 2019;9(1)

---

Entry by multiple picornaviruses is dependent on a pathway that includes TNK2, WASL, and NCK1

Jiang H, Leung C, Tahan S, Wang D
eLife 2019;8

---

Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages

Goddard P, Sanchez-Garrido J, Slater S, Kalyan M, Ruano-Gallego D, Marchès O, Fernández L, Frankel G, Shenoy A
Cell Reports 2019;27(4):1008-1017.e6

---

Identification of the PAK4 interactome reveals PAK4 phosphorylation of N-WASP and promotion of Arp2/3-dependent actin polymerization.

Zhao M, Spiess M, Johansson HJ, Olofsson H, Hu J, Lehtiö J, Strömblad S
Oncotarget 2017 Sep 29;8(44):77061-77074

---

N-wasp is required for stabilization of podocyte foot processes.

Schell C, Baumhakl L, Salou S, Conzelmann AC, Meyer C, Helmstädter M, Wrede C, Grahammer F, Eimer S, Kerjaschki D, Walz G, Snapper S, Huber TB
Journal of the American Society of Nephrology : JASN 2013 Apr;24(5):713-21

---

N-WASP coordinates the delivery and F-actin–mediated capture of MT1-MMP at invasive pseudopods

Yu X, Zech T, McDonald L, Gonzalez E, Li A, Macpherson I, Schwarz J, Spence H, Futó K, Timpson P, Nixon C, Ma Y, Anton I, Visegrády B, Insall R, Oien K, Blyth K, Norman J, Machesky L
Journal of Cell Biology 2012;199(3):527-544

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using HPA005750 antibody. Corresponding WASL RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more