H00002889-M01

antibody from Abnova Corporation

Targeting: RAPGEF1 C3G, GRF2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002889-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002889-M01, RRID:AB_464383

Product name

RAPGEF1 monoclonal antibody (M01), clone 3D10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RAPGEF1.

Antigen sequence

MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKL
MDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEA
TDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQ
KNLSW


Isotype

IgG

Antibody clone number

3D10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The WAVE2 complex regulates T cell receptor signaling to integrins via Abl- and CrkL-C3G-mediated activation of Rap1.

Nolz JC, Nacusi LP, Segovis CM, Medeiros RB, Mitchell JS, Shimizu Y, Billadeau DD
The Journal of cell biology 2008 Sep 22;182(6):1231-44

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 monoclonal antibody (M01), clone 3D10.Lane 1: RAPGEF1 transfected lysate(122.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RAPGEF1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol