Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-06500 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-06500, RRID:AB_1582352
- Product name
- Rabbit Polyclonal GRF2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRF2 Antibody [NBP1-06500] - Whole cell lysate from HeLa cells. C3G was also immunoprecipitated by rabbit anti-C3G antibody NBP1-06501.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRF2 Antibody [NBP1-06500] - Detection of Human C3G by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C3G antibody NBP1-06500 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GRF2 Antibody [NBP1-06500] - Detection of human C3G by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C3G antibody NBP1-06500 used for IP at 6 ug per reaction. C3G was also immunoprecipitated by rabbit anti-C3G antibody NBP1-06501. For blotting immunoprecipitated C3G, NBP1-06500 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.