Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-17070 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal KLF4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: KLF4 Antibody [NBP2-17070] - Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE gel, diluted at 1:3000.
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: KLF4 Antibody [NBP2-17070] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KLF4 Antibody [NBP2-17070] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:5000.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KLF4 Antibody [NBP2-17070] - HCT116 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:2000.
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: KLF4 Antibody [NBP2-17070] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KLF4 Antibody [NBP2-17070] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: KLF4 Antibody [NBP2-17070] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody..
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: KLF4 Antibody [NBP2-17070] - Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 ug of KLF4 antibody. Western blot analysis was performed using KLF4 antibody EasyBlot anti-Rabbit IgG) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - KLF4 antibody detects KLF4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - KLF4 antibody detects KLF4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - KLF4 antibody detects KLF4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: KLF4 Antibody [NBP2-17070] - Human embryonic stem cells, staining with KLF4 antibody at 1:100 dilution (purple) or rabbit IgG (black).