AP2725f

antibody from Abgent

Targeting: KLF4 EZF, GKLF

Antibody data

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Validation data

Product number: AP2725f - Provider product page
Provider: Abgent
Proper citation: Abgent Cat#AP2725f, RRID:AB_1967882
Product name: KLF4 Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 400 µl
Concentration: 0.42 mg/ml
Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

KLF4 protein structure - AP2725f shown in red.

Supportive validation

Submitted by: Abgent (provider)
Main image
Experimental details: "Immunofluorescent analysis of U251 cells, using KLF4 Antibody (Cat. #AP2725f). AP2725f was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red)."
Primary Ab dilution: 1:25

Supportive validation

Submitted by: Abgent (provider)
Main image
Experimental details: "Fluorescent confocal image of HeLa cells stained with AP2725f KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP2725f KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 ¼M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm."
Primary Ab dilution: 1:10~50

Supportive validation

Submitted by: Abgent (provider)
Main image
Experimental details: "Fluorescent confocal image of SY5Y cells stained with AP2725f KLF4 (S457) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP2725f KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 ¼M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei."
Primary Ab dilution: 1:10~50