GTX101509

antibody from GeneTex

Targeting: KLF4 EZF, GKLF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX101509

Provider

GeneTex

Proper citation

GeneTex Cat#GTX101509, RRID:AB_11173878

Product name

KLF4 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Kruppel-Like Factor 4 represses osteoblast differentiation via ciliary Hedgehog signaling.

Takeuchi Y, Kito A, Itoh S, Naruse H, Fujikawa J, Sadek KM, Akiyama S, Yamashiro T, Wakisaka S, Abe M
Experimental cell research 2018 Oct 15;371(2):417-425

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Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis.

Moon BS, Bai J, Cai M, Liu C, Shi J, Lu W
Nature communications 2018 Jan 26;9(1):401

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Involvement of CRF2 signaling in enterocyte differentiation.

Ducarouge B, Pelissier-Rota M, Powell R, Buisson A, Bonaz B, Jacquier-Sarlin M
World journal of gastroenterology 2017 Jul 28;23(28):5127-5145

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Cocktail of Chemical Compounds and Recombinant Proteins Robustly Promote the Stemness of Adipose-Derived Stem Cells.

Guo Y, Yu Q, Mathew S, Lian R, Xue Y, Cui Z, Li S, Zhu D, Han Y, Zeng Q, Liu S, Chen J
Cellular reprogramming 2017 Dec;19(6):363-371

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX101509 diluted at 1:500

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and KLF4 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and KLF4 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

KLF4 antibody detects KLF4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.Green: KLF4 protein stained by KLF4 antibody (GTX101509) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

KLF4 antibody detects KLF4 protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. KLF4 antibody (GTX101509) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

KLF4 antibody detects KLF4 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KLF4 antibody (GTX101509) dilution: 1:500.