IC3640A

antibody from Novus Biologicals

Targeting: KLF4 EZF, GKLF

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC3640A

Provider

Novus Biologicals

Product name

Goat Polyclonal KLF4 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross-reactivity with recombinant mouse (rm) KLF4 and less than 1% cross-reactivity with recombinant human (rh) KLF5, rhKLF6, and rmKLF15.

Reactivity

Human

Host

Goat

Conjugate

Red dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Molecular iodine inhibits the expression of stemness markers on cancer stem-like cells of established cell lines derived from cervical cancer.

Bigoni-Ordóñez GD, Ortiz-Sánchez E, Rosendo-Chalma P, Valencia-González HA, Aceves C, García-Carrancá A
BMC cancer 2018 Sep 26;18(1):928

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of KLF4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Goat Anti-Human KLF4 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3640A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.