Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-35303 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLF4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLF4 in HT29 cell lysate (35 µg/lane) using a KLF4 polyclonal antibody (Product # PA5-35303) at a dilution of 1:1000, followed by detection using an HRP-conjugated goat anti-rabbit IgG H&L secondary antibody at a 1:5000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KLF4 showing staining in the nucleus of SY5Y cells using a KLF4 polyclonal antibody (Product # PA5-35303) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (5.25 uM, 25 min). Nuclei were stained with Hoechst 33342 (blue) (10 µg/mL, 3 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KLF4 in formalin-fixed, paraffin-embedded human colon carcinoma using a KLF4 polyclonal antibody (Product # PA5-35303) followed by DAB staining.