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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324452 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal KLF4 Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal KLF4 Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KLF4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- KLF4 Antibody (C-term) (Cat. #TA324452) western blot analysis in A431,MCF-7 cell line lysates (35ug/lane).This demonstrates the KLF4 antibody detected the KLF4 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with TA324452 KLF4 (C-term) antibody. SY5Y cells were incubated with TA324452 KLF4 (C-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin . KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- KLF4 Antibody (C-term) (Cat. #TA324452) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
