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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- TA324722 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal KLF4 Antibody (N-term C74)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal KLF4 Antibody (N-term C74)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KLF4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of KLF4 Antibody (N-term C74) (Cat. #TA324722) in Jurkat,293 cell line lysates (35ug/lane).KLF4 (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of KLF4 Antibody (N-term C74) (Cat. #TA324722) in mouse stomach tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of HeLa cells stained with TA324722 KLF4 (N-term C74) antibody. HeLa cells were incubated with TA324722 KLF4 (N-term C74) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin . KLF4 immunoreactivity is localized to the nuclei in HeLa cells.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with TA324722 KLF4 (N-term C74) antibody. SY5Y cells were incubated with TA324722 KLF4 (N-term C74) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin . KLF4 immunoreactivity is localized to the nuclei in SY5Y cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with KLF4 antibody (N-term C74) (Cat.#TA324722), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human Spleen tissue reacted with KLF4 antibody (N-term C74) (Cat.#TA324722), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
