PAB29382

antibody from Abnova Corporation

Targeting: KLF4 EZF, GKLF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29382

Provider

Abnova Corporation

Product name

KLF4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human KLF4.

Antigen sequence

ETEEFNDLLDLDFILSNSLTHPPESVAATVSSSAS
ASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAP
GGTGGGLLYGRESAPPPTAPFNLADINDVSP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with KLF4 polyclonal antibody (Cat # PAB29382) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with KLF4 polyclonal antibody (Cat # PAB29382) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human skin with KLF4 polyclonal antibody (Cat # PAB29382) shows strong nuclear positivity in epidermal cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)