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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F41813 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- KLF4 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 394-421 from the human protein was used as the immunogen for this KLF4 antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the KLF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- KLF4 antibody western blot analysis in (1) A431 and (2) MCF-7 lysate. Predicted molecular weight: 50-60 kDa + ~75 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:200. KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- KLF4 antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
