Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
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Validation data
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- Product number
- ARG55837 - Provider product page
- Provider
- Arigo
- Product name
- anti-KLF4 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 481-504 of Human KLF4.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of MDA-MB231, NCI-H460 cell line lysates stained with ARG55837 anti-KLF4 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with ARG55837 anti-KLF4 antibody (green) at 1:100 dilution, 2 h at room temperature. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with ARG55837 anti-KLF4 antibody. Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: SY5Y cells stained with ARG55837 anti-KLF4 antibody (green) at 1:100 dilution, 2 h at room temperature. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with ARG55837 anti-KLF4 antibody. Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG55837 anti-KLF4 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Dylight Fluor? 554 (red) conjugated Phalloidin (red).