Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-21047 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-21047, RRID:AB_1642486
- Product name
- Mouse Monoclonal KLF4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - Lane 1. A549 cell lysate Lane 2. HeLa cell lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - Cell lysates of HeLa and A549 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KLF4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - The recombinant protein (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human KLF4 protein
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KLF4 Antibody (4E6) [NBP1-21047] - Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human KLF4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: KLF4 Antibody (4E6) [NBP1-21047]