A00120

antibody from Boster Biological Technology

Targeting: KLF4 EZF, GKLF

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00120

Provider

Boster Biological Technology

Product name

Anti-KLF4 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for KLF4 detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif.

Chen X, Wei X, Ma S, Xie H, Huang S, Yao M, Zhang L
Toxicology and applied pharmacology 2022 Dec 15;457:116319

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MicroRNA-92 regulates vascular smooth muscle cell function by targeting KLF4 during vascular restenosis and injury.

Deng S, Zhang Y, Wang Y, Lu X, Jiang Q
International journal of clinical and experimental pathology 2019;12(12):4253-4262

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SC1 Promotes MiR124-3p Expression to Maintain the Self-Renewal of Mouse Embryonic Stem Cells by Inhibiting the MEK/ERK Pathway.

Wei Q, Liu H, Ai Z, Wu Y, Liu Y, Shi Z, Ren X, Guo Z
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(5):2057-2072

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of KLF4 using anti-KLF4 antibody (A00120).KLF4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KLF4 Antibody (A00120) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image