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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- AM2142a - Provider product page
- Provider
- Abcepta
- Product name
- CD138 Antibody (C-term) (Ascites)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 100 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CD138 Antibody (C-term)(Ascites)(Cat. #AM2142a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).
- Primary Ab dilution
- 1:500~8000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites)(Cat#AM2142a) with U266 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites)(Cat#AM2142a) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CD138 Antibody (C-term) (Ascites) (Cat. #AM2142a) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis
- Primary Ab dilution
- 1:10~50
