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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF3190 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Syndecan-1/CD138 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Syndecan-1/CD138 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Syndecan-1 is observed and less than 1% cross-reactivity with rhSyndecan-2, recombinant mouse (rm) Syndecan-3 and rmSyndecan-4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Heparan sulfate proteoglycans present PCSK9 to the LDL receptor.
Gustafsen C, Olsen D, Vilstrup J, Lund S, Reinhardt A, Wellner N, Larsen T, Andersen CBF, Weyer K, Li JP, Seeberger PH, Thirup S, Madsen P, Glerup S
Nature communications 2017 Sep 11;8(1):503
Nature communications 2017 Sep 11;8(1):503
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Syndecan-1/CD138 in Mouse Kidney. Syndecan-1/CD138 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Syndecan-1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3190) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
