356526

antibody from BioLegend

Targeting: SDC1 CD138, SDC, SYND1, syndecan

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

356526

Provider

BioLegend

Proper citation

BioLegend Cat#356526, RRID:AB_2565510

Product name

Alexa Fluor® 594 anti-human CD138 (Syndecan-1)

Antibody type

Monoclonal

Antigen

A mixture of U266 and XG-1 human myeloma cell lines.

Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

MI15

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

U266B1 cells were fixed with 2% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml of anti-human CD138 Syndecan-1 (clone MI15) Alexa Fluor® 594 (red) in blocking buffer for two hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective.

Conjugate

Yellow dye