- 36-2900 - Invitrogen Antibodies SDC1 antibody

Submitted references Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.

Mirlekar B, Wang Y, Li S, Zhou M, Entwistle S, De Buysscher T, Morrison A, Herrera G, Harris C, Vincent BG, Ting JP, Rashid N, Kim WY, Yeh JJ, Pylayeva-Gupta Y
Cell reports. Medicine 2022 Sep 20;3(9):100744

Unusual bilateral kidney and duodenal plasmablastic lymphoma presentation in an elderly patient: A case report.

Liu YC, Su YT, Huang CK, Tsai YC, Chen YC, Li PF
Molecular and clinical oncology 2022 Jul;17(1):122

Transmembrane Protein TMEM230, a Target of Glioblastoma Therapy.

Cocola C, Magnaghi V, Abeni E, Pelucchi P, Martino V, Vilardo L, Piscitelli E, Consiglio A, Grillo G, Mosca E, Gualtierotti R, Mazzaccaro D, La Sala G, Di Pietro C, Palizban M, Liuni S, DePedro G, Morara S, Nano G, Kehler J, Greve B, Noghero A, Marazziti D, Bussolino F, Bellipanni G, D'Agnano I, Götte M, Zucchi I, Reinbold R
Frontiers in cellular neuroscience 2021;15:703431

Polarized Secretion of APRIL by the Tonsil Epithelium Upon Toll-Like Receptor Stimulation.

Sturm N, Quinterot M, Guyot JP, Righini C, Daamen WF, van Kuppevelt TH, Huard B
Frontiers in immunology 2021;12:715724

O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination.

Samart P, Luanpitpong S, Rojanasakul Y, Issaragrisil S
Journal of experimental & clinical cancer research : CR 2021 Mar 16;40(1):100

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

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Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts.

van de Vlekkert D, Qiu X, Annunziata I, d'Azzo A
Journal of visualized experiments : JoVE 2020 May 16;(159)

Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells.

Kumar-Singh A, Shrinet J, Parniewska MM, Fuxe J, Dobra K, Hjerpe A
Biomolecules 2020 Jul 11;10(7)

Skin-specific antibodies neutralizing mycolactone toxin during the spontaneous healing of Mycobacterium ulcerans infection.

Foulon M, Pouchin A, Manry J, Khater F, Robbe-Saule M, Durand A, Esnault L, Delneste Y, Jeannin P, Saint-André JP, Croué A, Altare F, Abel L, Alcaïs A, Marion E
Science advances 2020 Feb;6(9):eaax7781

SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines.

Farfán N, Orellana-Serradell O, Herrera D, Chrzanowsky D, Cubillos P, Marín G, Antonio García De Herreros A, Castellón EA, Contreras HR
International journal of molecular medicine 2020 Apr;45(4):1073-1080

Isolation, Purification and Characterization of Exosomes fromFibroblast Cultures of Skeletal Muscle.

Van De Vlekkert D, Qiu X, Annunziata I, d'Azzo A
Bio-protocol 2020 Apr 5;10(7):e3576

Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis.

van de Vlekkert D, Demmers J, Nguyen XX, Campos Y, Machado E, Annunziata I, Hu H, Gomero E, Qiu X, Bongiovanni A, Feghali-Bostwick CA, d'Azzo A
Science advances 2019 Jul;5(7):eaav3270

Neurotoxic reactive astrocytes are induced by activated microglia.

Liddelow SA, Guttenplan KA, Clarke LE, Bennett FC, Bohlen CJ, Schirmer L, Bennett ML, Münch AE, Chung WS, Peterson TC, Wilton DK, Frouin A, Napier BA, Panicker N, Kumar M, Buckwalter MS, Rowitch DH, Dawson VL, Dawson TM, Stevens B, Barres BA
Nature 2017 Jan 26;541(7638):481-487

Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation.

Allaire JM, Roy SA, Ouellet C, Lemieux É, Jones C, Paquet M, Boudreau F, Perreault N
International journal of cancer 2016 Jun 1;138(11):2700-12

A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.

Grau L, Luque-Garcia JL, González-Peramato P, Theodorescu D, Palou J, Fernandez-Gomez JM, Sánchez-Carbayo M
PloS one 2013;8(1):e53328

Syndecan 1 plays a novel role in enteral glutamine's gut-protective effects of the postischemic gut.

Peng Z, Ban K, Sen A, Grill R, Park P, Costantini TW, Kozar R
Shock (Augusta, Ga.) 2012 Jul;38(1):57-62

Heparan sulfate proteoglycans are required for cellular binding of the hepatitis E virus ORF2 capsid protein and for viral infection.

Kalia M, Chandra V, Rahman SA, Sehgal D, Jameel S
Journal of virology 2009 Dec;83(24):12714-24

Primary human glomerular endothelial cells produce proteoglycans, and puromycin affects their posttranslational modification.

Björnson A, Moses J, Ingemansson A, Haraldsson B, Sörensson J
American journal of physiology. Renal physiology 2005 Apr;288(4):F748-56

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Supportive validation

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Experimental details: Western blot analysis of (A) SW480, (B) T47D, and (C) PC-3 cell lysates using Syndecan-1 polyclonal antibody, rabbit (Product # 36-2900).

Supportive validation

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Experimental details: Western blot analysis of Syndecan-1 was performed by loading 20 µg of MDA-MB-231 (lane1) and Caco-2 (lane2) lysate using Novex®NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4°C overnight. Syndecan-1 was detected at ~ 31 kDa using Syndecan-1 Rabbit Polyclonal Antibody (Product # 36-2900) at 1-2 µg/mL in 5% skim milk for 3 hour at room temperature on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical staining of human bone marrow using Syndecan-1 polyclonal antibody, rabbit (PAD: ZMD.289) (Product # 36-2900).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Syndecan-1 showing staining in the cytoplasm and membrane of paraffin-embedded human skin tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Syndecan-1 polyclonal antibody (Product # 36-2900) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Syndecan-1/CD138 showing staining in the cytoplasm and membrane of paraffin-embedded human skin tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Syndecan-1/CD138 polyclonal antibody (Product # 36-2900) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Syndecan-1 showing staining in the cytoplasm and membrane of paraffin-embedded human small intestine tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Syndecan-1 polyclonal antibody (Product # 36-2900) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometry analysis of Syndecan-1 was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Tritonª X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Syndecan-1 Rabbit Polyclonal Antibody (362900, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor¨ 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Inhibition of Ca 2+ influx channels and hyper- O -GlcNAcylation impairs MM dissemination in vivo. a-e Luciferase-labeled sgTRPM7 or WT RPMI8226 cells were injected into NSG mice via tail vein. A Representative bioluminescence imaging of mice taken at the time of inoculation (week 0) and weekly post-injection (week 1, 2, 3 and 4). b, c Quantification of whole-body luminescence signals normalized to their initial signal at week 0 ( b ) and relative to WT control cells ( c ). Data mean +- SD ( n = 5). *** P < 0.001 versus WT cells; two-tailed Student's t -test. d Representative ex vivo imaging from isolated internal organs including brain, lung, kidney, bone, heart, spleen, and liver at week 4 post-injection (see also Additional file 2 : Fig. S15 for quantification of ex vivo IVIS images). e Representative IHC micrographs of femur bone and liver sections staining for human CD138 (see also Additional file 2 : Fig. S17 for additional IHC micrographs). Scale bar = 100 mum. f-j Luciferase-labeled sgMGEA5 or WT RPMI8226 cells were similarly injected into NSG mice via tail vein and bioluminescence imaging and IHC staining were similarly performed as described in A-E (see also Additional file 2 : Fig. S18 for additional IHC micrographs)

Supportive validation

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Experimental details: Figure 2 The HSPG syndecan-1 and -4 are expressed by tonsil epithelial cells. (A) Serial sections of tonsils were stained for native HS chain (10e4 reactivity), the HS stub (3g10 reactivity), and syndecan-1. Scale bar = 10 um. (B) Tonsil sections were costained for 10e4 (green)/syndecan-1 (red) and syndecan-4 (green)/syndecan-1 (red). Scale bar = 5 um. The surface epithelium is shown. In some figures, the epithelium (epi.) and sub-epithelium (sub-epi.) are marked. The arrow and box show the basement membrane and a plasmocyte, respectively. Results are representative of more than 15 tonsillectomies for patients with recurrent infections.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 4 Emergence of plasma cells (CD138 + ) in the skin during M. ulcerans infection in the FVB/N mouse model of spontaneous healing. ( A ) Cells were analyzed by four-color flow cytometry with the specific staining of total lymphoid cells (CD45 + ), B cells (population P1 = CD45 + , CD19 + , B220 + ), plasmablasts (population P2 = CD45 + , CD19 + , B220 int ), and plasma cells (CD45 + , CD19 - , B220 int , CD138 + ). The results shown here are from one representative experiment performed in duplicate. ( B ) Local inflammatory infiltration (inset) in the dermis of M. ulcerans infected mice at the ulcerative stage, typical of M. ulcerans infection. ep, epidermis. Immunostaining showing CD138 + plasma cells (activated B cells able to produce antibodies) in the dermis. ( C ) Flow cytometry analysis showing the emergence of plasma cells (CD45 + , CD19 - , B220 int , CD138 + ) during the early steps of the spontaneous healing process (the control is uninfected skin). The histograms show the means +- SD. * P < 0.05 (Student's t test). Scale bars, 160 mum (B top), 80 mum (B, top, inset), 100 mum (B bottom) and 20 mum (B bottom, inset).

Supportive validation

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Experimental details: Fig. 4 Neu1 -/- exosomes contain increased levels of components of the TGF-beta and WNT signaling pathways. ( A ) Exosomes exocytosed by Neu1 -/- myofibroblasts were verified on immunoblots probed with antibodies against established exosomal markers [Alix, CD81, CD9, flottilin-1 (Flot1), Syndecan-1 (SDC1), and syntenin 1 (SDCBP1)]. ( B ) Immunoblot analyses of WT and Neu1 -/- exosomes performed with antibodies against canonical (beta-catenin, GSK3beta, LRP5, and WNT3a) and noncanonical (WNT5a/b) WNT signaling. ( C and D ) Immunoblots of WT and Neu1 -/- exosomes probed with anti-TGF-beta1 (C) and LAP (D) antibodies. ( E ) Exosomes treated with proteinase K were analyzed on immunoblots probed with anti-TGF-beta1, anti-LAP, anti-WNT5a/b, and anti-beta-catenin antibodies.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Immunohistochemistry in benign prostatic hyperplasia and prostate cancer samples. Localization of (A) SNAIL, (C) SLUG, (E) ED-SDC-1 and (G) ID-SDC-1. (G) Nuclear ID-SDC-1 (black arrows) and magnification (rectangle in the center of the image) are included in the lower right corner. Hematoxylin staining (negative control) is presented in the upper left corner. (B) SNAIL (P=0.0011), (D) SLUG (P=0.0004), (F) ED-SDC-1 (P=0.0011) and (H) ID-SDC-1 (P=0.0004) protein levels were quantified. The data represent the average of 3 independent experiments, and the data are presented as the mean +- standard error of the mean. Data were analyzed using a Student's t-test. ** P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 ID-SDC-1 and ED-SDC-1 location in PC3 cells with ectopic SNAIL or SLUG expression. (A and C) Western blot analysis of SNAIL, SLUG, vimentin and E-cadherin protein levels. (B and D) Quantification of the western blot analysis data. Data were analyzed using a Student's t-test. (E) Confocal microscopy of DAPI (nuclei), RFP (transduction control) and ID-SDC-1 (green) in EV, SNAIL or SLUG cells. Scale bar=10 u m. (F) Nuclear region magnification. Scale bar=10 u m. (G) Nuclear ID-SDC-1 quantification (integrated optical density per area, arbitrary units). Data were analyzed using ANOVA followed by a Tukey post hoc test. (H) ID-SDC-1 with DAPI Manders' overlap coefficient. Data were analyzed using ANOVA followed by a Tukey post hoc test. (I) Confocal microscopy of DAPI (nuclei), RFP (transduction control), ED-SDC-1 (green) and CD44 (blue) in the EV, SNAIL or SLUG cells. Scale bar=10 u m. (J) ED-SDC-1 with CD44 Manders' overlap coefficient. Data were analyzed using ANOVA followed by a Tukey post hoc test. The data represent the average of three independent experiments and are presented as the mean +- standard error of the mean. * P

36-2900

Antibody data