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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB2966 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Syndecan-1/CD138 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Syndecan-1/CD138 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Syndecan-1, recombinant mouse (rm) Syndecan-3 or rmSyndecan-4 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P18828- Isotype
- IgG
- Antibody clone number
- 300506
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
Kowalewska PM, Patrick AL, Fox-Robichaud AE
PloS one 2014;9(9):e104537
PloS one 2014;9(9):e104537
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Syndecan-1/CD138 in NMuMG Mouse Cell Line. Syndecan-1/CD138 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using Rat Anti-Mouse Syndecan-1/CD138 Monoclonal Antibody (Catalog # MAB2966) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
