Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- MA5-12400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD138 Monoclonal Antibody (MI15)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-12400 targets CD138 in IHC (P), FACS, IP, and WB applications and shows reactivity with Human and Rat samples.
- Antibody clone number
- MI15
- Concentration
- Conc. Not Determined
Submitted references The Role of Chaperone-Mediated Autophagy in Bortezomib Resistant Multiple Myeloma.
Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells.
EBV+ diffuse large B-cell lymphoma arising within atrial myxoma. An example of a distinct primary cardiac EBV+ DLBCL of immunocompetent patients.
Activated B cells in the granulomas of nonhuman primates infected with Mycobacterium tuberculosis.
Primary myoepithelial carcinoma of the larynx: case report and review of the literature.
Differences in the tumor microenvironment between African-American and European-American breast cancer patients.
An immunohistochemical study of colon adenomas and carcinomas: E-cadherin, Syndecan-1, Ets-1.
Local immune alterations in oral mucosa of the edentulous patients; possible cause of teeth loss?
Nikesitch N, Rebeiro P, Ho LL, Pothula S, Wang XM, Khong T, Quek H, Spencer A, Lee CS, Roberts TL, Ling SCW
Cells 2021 Dec 8;10(12)
Cells 2021 Dec 8;10(12)
Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells.
Kumar-Singh A, Shrinet J, Parniewska MM, Fuxe J, Dobra K, Hjerpe A
Biomolecules 2020 Jul 11;10(7)
Biomolecules 2020 Jul 11;10(7)
EBV+ diffuse large B-cell lymphoma arising within atrial myxoma. An example of a distinct primary cardiac EBV+ DLBCL of immunocompetent patients.
Svec A, Rangaiah M, Giles M, Jaksa R, McAulay KA
Pathology, research and practice 2012 Mar 15;208(3):172-6
Pathology, research and practice 2012 Mar 15;208(3):172-6
Activated B cells in the granulomas of nonhuman primates infected with Mycobacterium tuberculosis.
Phuah JY, Mattila JT, Lin PL, Flynn JL
The American journal of pathology 2012 Aug;181(2):508-14
The American journal of pathology 2012 Aug;181(2):508-14
Primary myoepithelial carcinoma of the larynx: case report and review of the literature.
Yu G, Qu G, Kong L, Pan X, Wang W, Lv J
Pathology, research and practice 2011 Feb 15;207(2):127-30
Pathology, research and practice 2011 Feb 15;207(2):127-30
Differences in the tumor microenvironment between African-American and European-American breast cancer patients.
Martin DN, Boersma BJ, Yi M, Reimers M, Howe TM, Yfantis HG, Tsai YC, Williams EH, Lee DH, Stephens RM, Weissman AM, Ambs S
PloS one 2009;4(2):e4531
PloS one 2009;4(2):e4531
An immunohistochemical study of colon adenomas and carcinomas: E-cadherin, Syndecan-1, Ets-1.
Pap Z, Pávai Z, Dénes L, Kovalszky I, Jung J
Pathology oncology research : POR 2009 Dec;15(4):579-87
Pathology oncology research : POR 2009 Dec;15(4):579-87
Local immune alterations in oral mucosa of the edentulous patients; possible cause of teeth loss?
Zurac S, Girtan M, Lavric L, Petsakos G, Stăniceanu F, Bastian A, Andrei R, Popp C, Laba E
Romanian journal of internal medicine = Revue roumaine de medecine interne 2008;46(3):249-53
Romanian journal of internal medicine = Revue roumaine de medecine interne 2008;46(3):249-53
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with CD138 antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of plasma cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD138 in PC12 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD138 monoclonal antibody (Product # MA5-12400) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD138 in Ramos cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD138 monoclonal antibody (Product # MA5-12400) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD138 in U266 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD138 monoclonal antibody (Product # MA5-12400) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.