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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunohistochemistry [2]
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- Product number
- AF2780 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Syndecan-1/CD138 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4, and recombinant mouse Syndecan-1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_002988- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Augmented concentrations of CX3CL1 are associated with interstitial lung disease in systemic sclerosis.
GWAS for Interleukin-1β levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation.
Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device.
Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.
Shed syndecan-1 translocates to the nucleus of cells delivering growth factors and inhibiting histone acetylation: a novel mechanism of tumor-host cross-talk.
Novel processed form of syndecan-1 shed from SCC-9 cells plays a role in cell migration.
Heparanase plays a dual role in driving hepatocyte growth factor (HGF) signaling by enhancing HGF expression and activity.
CD28-mediated regulation of multiple myeloma cell proliferation and survival.
Hoffmann-Vold AM, Weigt SS, Palchevskiy V, Volkmann E, Saggar R, Li N, Midtvedt Ø, Lund MB, Garen T, Fishbein MC, Ardehali A, Ross DJ, Ueland T, Aukrust P, Lynch JP 3rd, Elashoff RM, Molberg Ø, Belperio JA
PloS one 2018;13(11):e0206545
PloS one 2018;13(11):e0206545
GWAS for Interleukin-1β levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation.
Offenbacher S, Jiao Y, Kim SJ, Marchesan J, Moss KL, Jing L, Divaris K, Bencharit S, Agler CS, Morelli T, Zhang S, Sun L, Seaman WT, Cowley D, Barros SP, Beck JD, Munz M, Schaefer AS, North KE
Nature communications 2018 Sep 11;9(1):3686
Nature communications 2018 Sep 11;9(1):3686
Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device.
Qasaimeh MA, Wu YC, Bose S, Menachery A, Talluri S, Gonzalez G, Fulciniti M, Karp JM, Prabhala RH, Karnik R
Scientific reports 2017 Apr 4;7:45681
Scientific reports 2017 Apr 4;7:45681
Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.
Purushothaman A, Bandari SK, Liu J, Mobley JA, Brown EE, Sanderson RD
The Journal of biological chemistry 2016 Jan 22;291(4):1652-63
The Journal of biological chemistry 2016 Jan 22;291(4):1652-63
Shed syndecan-1 translocates to the nucleus of cells delivering growth factors and inhibiting histone acetylation: a novel mechanism of tumor-host cross-talk.
Stewart MD, Ramani VC, Sanderson RD
The Journal of biological chemistry 2015 Jan 9;290(2):941-9
The Journal of biological chemistry 2015 Jan 9;290(2):941-9
Novel processed form of syndecan-1 shed from SCC-9 cells plays a role in cell migration.
Aragão AZ, Belloni M, Simabuco FM, Zanetti MR, Yokoo S, Domingues RR, Kawahara R, Pauletti BA, Gonçalves A, Agostini M, Graner E, Coletta RD, Fox JW, Paes Leme AF
PloS one 2012;7(8):e43521
PloS one 2012;7(8):e43521
Heparanase plays a dual role in driving hepatocyte growth factor (HGF) signaling by enhancing HGF expression and activity.
Ramani VC, Yang Y, Ren Y, Nan L, Sanderson RD
The Journal of biological chemistry 2011 Feb 25;286(8):6490-9
The Journal of biological chemistry 2011 Feb 25;286(8):6490-9
CD28-mediated regulation of multiple myeloma cell proliferation and survival.
Bahlis NJ, King AM, Kolonias D, Carlson LM, Liu HY, Hussein MA, Terebelo HR, Byrne GE Jr, Levine BL, Boise LH, Lee KP
Blood 2007 Jun 1;109(11):5002-10
Blood 2007 Jun 1;109(11):5002-10
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Syndecan-1/CD138 in Human Jejunum. Syndecan-1/CD138 was detected in immersion fixed paraffin-embedded sections of human jejunum using Human Syndecan-1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Syndecan-1/CD138 in Human Ileum. Syndecan-1/CD138 was detected in immersion fixed paraffin-embedded sections of human ileum using Human Syndecan-1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
