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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- GTX105155 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105155, RRID:AB_11167407
- Product name
- ETFA antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE GTX105155 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ETFA antibody detects ETFA protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract10 % SDS-PAGEETFA antibody (GTX105155) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX105155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ETFA(GTX105155) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 £gg of ETFA antibody (GTX105155).Western blot analysis was performed using ETFA antibody (GTX105155).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ETFA(GTX105155) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA antibody (GTX105155) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ETFA antibody (GTX105155) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody (GTX105155) dilution: 1:500.
